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. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and <t>CoxIV</t> antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).
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. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and <t>CoxIV</t> antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).
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. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and <t>CoxIV</t> antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).
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. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and <t>CoxIV</t> antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).
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. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and <t>CoxIV</t> antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).
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. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and <t>CoxIV</t> antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).
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. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and <t>CoxIV</t> antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).
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. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and <t>CoxIV</t> antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).
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. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and <t>CoxIV</t> antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).
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. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and <t>CoxIV</t> antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).
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Image Search Results


. Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and CoxIV antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).

Journal: PLoS ONE

Article Title: Shifting the Paradigm: The Putative Mitochondrial Protein ABCB6 Resides in the Lysosomes of Cells and in the Plasma Membrane of Erythrocytes

doi: 10.1371/journal.pone.0037378

Figure Lengend Snippet: . Expression of the endogenous ABCB6 protein in K562 cells was visualized by the monoclonal ABCB6-567 antibody (green), lysosomes and mitochondria were labeled with LAMP1 (red, lower panel) and CoxIV antibodies (red, upper panel), respectively. ABCB6 colocalizes with LAMP1 (yellow on the overlay, r = 0.79), but not with CoxIV (r = 0.14). B . Flag-tagged ABC proteins were expressed following transient transfection of HeLa cells. The cDNA-derived ABC transporters were visualized with an anti-FLAG tag antibody (green, left panel); mitochondria were labeled with CoxIV (red, middle panel). Whereas the canonical mitochondrial ABC transporters are confined to the mitochondria (merged image, right panel, r = 0.87, r = 0.7, r = 0.84 for ABCB7, ABCB8 and ABCB10, respectively), ABCB6 does not show colocalisation with the mitochondrial marker (merged image, right panel, r = 0.13).

Article Snippet: The following primary antibodies were used for confocal microscopy: anti-Flag (F3165, Sigma); panCadherin (Ab16505, Abcam); giantin (Ab24586, Abcam); Lamp1 (L1418, Sigma); LAMP1 (611043, BD Bioscience); calnexin and CoxIV (Cellular Localization IF Antibody Sampler Kit, Cell Signaling, #4753).

Techniques: Expressing, Labeling, Transfection, Derivative Assay, FLAG-tag, Marker